v    Principle:-

 The test organisms are cultured in MIU medium (or in Christensen’s urea broth). If the strain produces urease, it acts on urea, and ammonium carbonate is formed with the release of ammonia. The medium becomes alkaline and the color of the medium changes to red pink.

v  Requirements:-

1.      Motility Indole Urea (MIU) medium.

2.       Test tubes (15 x 125 mm)

v  Procedure:-

1.      Inoculate MIU medium with acolony of the test organism.

2.      Incubate at 35°C overnight.

3.      Examine the medium by looking for a red pink color.

v    Observations:-

1.     Red-pink medium       :  Positive test

2.     No red pink color        :  Negative test

v  Quality control:-

   Use the following microorganisms to confirm the reliability of reagents:-

   Positive control     :           Klebsiella aerogenes

   Negative control    :         E. coli